1ahp

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[[Image:1ahp.gif|left|200px]]<br /><applet load="1ahp" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ahp.gif|left|200px]]
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caption="1ahp, resolution 3.0&Aring;" />
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'''OLIGOSACCHARIDE SUBSTRATE BINDING IN ESCHERICHIA COLI MALTODEXTRIN PHSPHORYLASE'''<br />
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{{Structure
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|PDB= 1ahp |SIZE=350|CAPTION= <scene name='initialview01'>1ahp</scene>, resolution 3.0&Aring;
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|SITE= <scene name='pdbsite=1:Catalytic+Site+Is+Located+At+The+Base+Of+A+Cleft+Between+...'>1</scene>
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|LIGAND= <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphorylase Phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.1 2.4.1.1]
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|GENE=
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}}
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'''OLIGOSACCHARIDE SUBSTRATE BINDING IN ESCHERICHIA COLI MALTODEXTRIN PHSPHORYLASE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1AHP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MAL:'>MAL</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=PLP:'>PLP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphorylase Phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.1 2.4.1.1] Known structural/functional Site: <scene name='pdbsite=1:Catalytic+Site+Is+Located+At+The+Base+Of+A+Cleft+Between+...'>1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHP OCA].
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1AHP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHP OCA].
==Reference==
==Reference==
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Oligosaccharide substrate binding in Escherichia coli maltodextrin phosphorylase., O'Reilly M, Watson KA, Schinzel R, Palm D, Johnson LN, Nat Struct Biol. 1997 May;4(5):405-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9145112 9145112]
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Oligosaccharide substrate binding in Escherichia coli maltodextrin phosphorylase., O'Reilly M, Watson KA, Schinzel R, Palm D, Johnson LN, Nat Struct Biol. 1997 May;4(5):405-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9145112 9145112]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Phosphorylase]]
[[Category: Phosphorylase]]
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[[Category: substrate]]
[[Category: substrate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:44:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:57:40 2008''

Revision as of 07:57, 20 March 2008

Image:1ahp.gif


PDB ID 1ahp

Drag the structure with the mouse to rotate
, resolution 3.0Å
Sites:
Ligands: , , and
Activity: Phosphorylase, with EC number 2.4.1.1
Coordinates: save as pdb, mmCIF, xml



OLIGOSACCHARIDE SUBSTRATE BINDING IN ESCHERICHIA COLI MALTODEXTRIN PHSPHORYLASE


Overview

The crystal structure of E. coli maltodextrin phosphorylase co-crystallized with an oligosaccharide has been solved at 3.0 A resolution, providing the first structure of an oligosaccharide bound at the catalytic site of an alpha-glucan phosphorylase. An induced fit mechanism brings together two domains across the catalytic site tunnel. A stacking interaction between the glucosyl residue and the aromatic group of a tyrosine residue at a sub-site remote (8 A) from the catalytic site provides a key element in substrate recognition; mutation of this residue to Ala decreases the Kcat/Km by 10(4). Extrapolation of the results to substrate binding across the site of attack by phosphorolysis indicates a likely alteration in the glycosidic torsion angles from their preferred values, an alteration that appears to be important for the catalytic mechanism.

About this Structure

1AHP is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Oligosaccharide substrate binding in Escherichia coli maltodextrin phosphorylase., O'Reilly M, Watson KA, Schinzel R, Palm D, Johnson LN, Nat Struct Biol. 1997 May;4(5):405-12. PMID:9145112

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