1pvs
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=7HP:7-HYDROXY-PYRAZOLO[4,3-D]PYRIMIDINE'>7HP</scene> | |LIGAND= <scene name='pdbligand=7HP:7-HYDROXY-PYRAZOLO[4,3-D]PYRIMIDINE'>7HP</scene> | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] </span> |
|GENE= ALKA OR AIDA OR B2068 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= ALKA OR AIDA OR B2068 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pvs OCA], [http://www.ebi.ac.uk/pdbsum/1pvs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pvs RCSB]</span> | ||
}} | }} | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Teale, M.]] | [[Category: Teale, M.]] | ||
| - | [[Category: 7HP]] | ||
[[Category: alka]] | [[Category: alka]] | ||
[[Category: dna glycosylase]] | [[Category: dna glycosylase]] | ||
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[[Category: reaction-product complex]] | [[Category: reaction-product complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:05:13 2008'' |
Revision as of 20:05, 30 March 2008
| |||||||
| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | ALKA OR AIDA OR B2068 (Escherichia coli) | ||||||
| Activity: | DNA-3-methyladenine glycosylase II, with EC number 3.2.2.21 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex
Overview
Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II (AlkA) functions primarily by removing alkylation damage from duplex and single stranded DNA. A crystal structure of AlkA was refined to 2.0 A resolution. This structure in turn was used to refine an AlkA-hypoxanthine (substrate) complex structure to 2.4 A resolution. The complex structure shows hypoxanthine located in AlkA's active site stacked between residues W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity.
About this Structure
1PVS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
3-methyladenine-DNA glycosylase II: the crystal structure of an AlkA-hypoxanthine complex suggests the possibility of product inhibition., Teale M, Symersky J, DeLucas L, Bioconjug Chem. 2002 May-Jun;13(3):403-7. PMID:12009927
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