1a55

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[[Image:1a55.gif|left|200px]]
[[Image:1a55.gif|left|200px]]
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{{Structure
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|PDB= 1a55 |SIZE=350|CAPTION= <scene name='initialview01'>1a55</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1a55", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=2HP:Ligands+Of+2hp+(H2po4)+Ion+322'>2HP</scene>
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|LIGAND= <scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|GENE= PHO-S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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|DOMAIN=
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{{STRUCTURE_1a55| PDB=1a55 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a55 OCA], [http://www.ebi.ac.uk/pdbsum/1a55 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a55 RCSB]</span>
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'''PHOSPHATE-BINDING PROTEIN MUTANT A197C'''
'''PHOSPHATE-BINDING PROTEIN MUTANT A197C'''
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[[Category: Vasisht, N.]]
[[Category: Vasisht, N.]]
[[Category: Webb, M R.]]
[[Category: Webb, M R.]]
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[[Category: phosphotransferase]]
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[[Category: Phosphotransferase]]
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[[Category: transport]]
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[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:49:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:33:28 2008''
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Revision as of 06:49, 2 May 2008

Image:1a55.gif

Template:STRUCTURE 1a55

PHOSPHATE-BINDING PROTEIN MUTANT A197C


Overview

Crystal structures are presented for the A197C mutant of Escherichia coli phosphate binding protein (PBP) and the same mutant labeled at Cys197 with N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Both proteins are complexed with inorganic phosphate. The latter molecule, MDCC-PBP, exhibits a large increase in fluorescence on binding inorganic phosphate. The resulting high-fluorescence state of the coumarin and the ability of this coumarin to monitor the conformational changes associated with inorganic phosphate binding are interpreted in terms of the specific interactions of MDCC with the protein. The structure helps to explain why this particular label gives a high-fluorescence state on binding inorganic phosphate, while several other related labels do not, and hence aids our general understanding of environmentally sensitive fluorescence probes on proteins.

About this Structure

1A55 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate., Hirshberg M, Henrick K, Haire LL, Vasisht N, Brune M, Corrie JE, Webb MR, Biochemistry. 1998 Jul 21;37(29):10381-5. PMID:9671506 Page seeded by OCA on Fri May 2 09:49:39 2008

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