1ahp

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[[Image:1ahp.gif|left|200px]]
[[Image:1ahp.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ahp |SIZE=350|CAPTION= <scene name='initialview01'>1ahp</scene>, resolution 3.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1ahp", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=1:Catalytic+Site+Is+Located+At+The+Base+Of+A+Cleft+Between+...'>1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphorylase Phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.1 2.4.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1ahp| PDB=1ahp | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ahp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahp OCA], [http://www.ebi.ac.uk/pdbsum/1ahp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ahp RCSB]</span>
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}}
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'''OLIGOSACCHARIDE SUBSTRATE BINDING IN ESCHERICHIA COLI MALTODEXTRIN PHSPHORYLASE'''
'''OLIGOSACCHARIDE SUBSTRATE BINDING IN ESCHERICHIA COLI MALTODEXTRIN PHSPHORYLASE'''
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[[Category: Schinzel, R.]]
[[Category: Schinzel, R.]]
[[Category: Watson, K A.]]
[[Category: Watson, K A.]]
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[[Category: ecoli]]
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[[Category: Ecoli]]
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[[Category: induced-fit]]
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[[Category: Induced-fit]]
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[[Category: maltodextrin]]
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[[Category: Maltodextrin]]
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[[Category: oligosaccharide]]
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[[Category: Oligosaccharide]]
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[[Category: phosphorylase]]
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[[Category: Phosphorylase]]
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[[Category: stacking]]
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[[Category: Stacking]]
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[[Category: substrate]]
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[[Category: Substrate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:17:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:40:25 2008''
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Revision as of 07:17, 2 May 2008

Image:1ahp.gif

Template:STRUCTURE 1ahp

OLIGOSACCHARIDE SUBSTRATE BINDING IN ESCHERICHIA COLI MALTODEXTRIN PHSPHORYLASE


Overview

The crystal structure of E. coli maltodextrin phosphorylase co-crystallized with an oligosaccharide has been solved at 3.0 A resolution, providing the first structure of an oligosaccharide bound at the catalytic site of an alpha-glucan phosphorylase. An induced fit mechanism brings together two domains across the catalytic site tunnel. A stacking interaction between the glucosyl residue and the aromatic group of a tyrosine residue at a sub-site remote (8 A) from the catalytic site provides a key element in substrate recognition; mutation of this residue to Ala decreases the Kcat/Km by 10(4). Extrapolation of the results to substrate binding across the site of attack by phosphorolysis indicates a likely alteration in the glycosidic torsion angles from their preferred values, an alteration that appears to be important for the catalytic mechanism.

About this Structure

1AHP is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Oligosaccharide substrate binding in Escherichia coli maltodextrin phosphorylase., O'Reilly M, Watson KA, Schinzel R, Palm D, Johnson LN, Nat Struct Biol. 1997 May;4(5):405-12. PMID:9145112 Page seeded by OCA on Fri May 2 10:17:07 2008

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