1a55

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[[Image:1a55.gif|left|200px]]
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{{STRUCTURE_1a55| PDB=1a55 | SCENE= }}
{{STRUCTURE_1a55| PDB=1a55 | SCENE= }}
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'''PHOSPHATE-BINDING PROTEIN MUTANT A197C'''
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===PHOSPHATE-BINDING PROTEIN MUTANT A197C===
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==Overview==
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Crystal structures are presented for the A197C mutant of Escherichia coli phosphate binding protein (PBP) and the same mutant labeled at Cys197 with N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Both proteins are complexed with inorganic phosphate. The latter molecule, MDCC-PBP, exhibits a large increase in fluorescence on binding inorganic phosphate. The resulting high-fluorescence state of the coumarin and the ability of this coumarin to monitor the conformational changes associated with inorganic phosphate binding are interpreted in terms of the specific interactions of MDCC with the protein. The structure helps to explain why this particular label gives a high-fluorescence state on binding inorganic phosphate, while several other related labels do not, and hence aids our general understanding of environmentally sensitive fluorescence probes on proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 9671506 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9671506}}
==About this Structure==
==About this Structure==
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[[Category: Phosphotransferase]]
[[Category: Phosphotransferase]]
[[Category: Transport]]
[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:49:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:09:06 2008''

Revision as of 13:09, 30 June 2008

Image:1a55.png

Template:STRUCTURE 1a55

PHOSPHATE-BINDING PROTEIN MUTANT A197C

Template:ABSTRACT PUBMED 9671506

About this Structure

1A55 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate., Hirshberg M, Henrick K, Haire LL, Vasisht N, Brune M, Corrie JE, Webb MR, Biochemistry. 1998 Jul 21;37(29):10381-5. PMID:9671506

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