1st8

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Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus

Structural highlights

1st8 is a 1 chain structure with sequence from Cichorium intybus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.35Å
Ligands:GOL, MAN, NAG
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q93X60_CICIN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Fructan 1-exohydrolase, an enzyme involved in fructan degradation, belongs to the glycosyl hydrolase family 32. The structure of isoenzyme 1-FEH IIa from Cichorium intybus is described at a resolution of 2.35 A. The structure consists of an N-terminal fivefold beta-propeller domain connected to two C-terminal beta-sheets. The putative active site is located entirely in the beta-propeller domain and is formed by amino acids which are highly conserved within glycosyl hydrolase family 32. The fructan-binding site is thought to be in the cleft formed between the two domains. The 1-FEH IIa structure is compared with the structures of two homologous but functionally different enzymes: a levansucrase from Bacillus subtilis (glycosyl hydrolase family 68) and an invertase from Thermotoga maritima (glycosyl hydrolase family 32).

X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus.,Verhaest M, Ende WV, Roy KL, De Ranter CJ, Laere AV, Rabijns A Plant J. 2005 Feb;41(3):400-11. PMID:15659099[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
4 reviews cite this structure
Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications.
Lammens et al. (2009)
3 more
55 other articles
No citations found

References

  1. ↑ Verhaest M, Ende WV, Roy KL, De Ranter CJ, Laere AV, Rabijns A. X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus. Plant J. 2005 Feb;41(3):400-11. PMID:15659099 doi:http://dx.doi.org/10.1111/j.1365-313X.2004.02304.x

Contents


PDB ID 1st8

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