3f73

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Alignment of guide-target seed duplex within an argonaute silencing complex

Structural highlights

3f73 is a 6 chain structure with sequence from Thermus thermophilus HB27. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:MG, PO4
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q746M7_THET2

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Here we report on a 3.0 A crystal structure of a ternary complex of wild-type Thermus thermophilus argonaute bound to a 5'-phosphorylated 21-nucleotide guide DNA and a 20-nucleotide target RNA containing cleavage-preventing mismatches at the 10-11 step. The seed segment (positions 2 to 8) adopts an A-helical-like Watson-Crick paired duplex, with both ends of the guide strand anchored in the complex. An arginine, inserted between guide-strand bases 10 and 11 in the binary complex, locking it in an inactive conformation, is released on ternary complex formation. The nucleic-acid-binding channel between the PAZ- and PIWI-containing lobes of argonaute widens on formation of a more open ternary complex. The relationship of structure to function was established by determining cleavage activity of ternary complexes containing position-dependent base mismatch, bulge and 2'-O-methyl modifications. Consistent with the geometry of the ternary complex, bulges residing in the seed segments of the target, but not the guide strand, were better accommodated and their complexes were catalytically active.

Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex.,Wang Y, Juranek S, Li H, Sheng G, Tuschl T, Patel DJ Nature. 2008 Dec 18;456(7224):921-6. PMID:19092929[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
82 reviews cite this structure
Carthew et al. (2009)
No citations found

See Also

References

  1. Wang Y, Juranek S, Li H, Sheng G, Tuschl T, Patel DJ. Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex. Nature. 2008 Dec 18;456(7224):921-6. PMID:19092929 doi:nature07666

Contents


PDB ID 3f73

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