Structural highlights
Publication Abstract from PubMed
The hairpin ribozyme is a small catalytic RNA with a unique two-domain structure. Here we present the solution structure of the loop B domain of the hairpin ribozyme, which contains most of the catalytically essential nucleotides. The 38-nucleotide domain contains a 16-nucleotide internal loop that forms one of the largest non-Watson-Crick segments of base pairing thus far determined by either NMR or crystallography. Since the solution structure of the smaller loop A domain has been previously solved, an NMR structure-based model of the 22,000 Mr hairpin ribozyme-substrate open complex emerges by joining the two domain structures. Strikingly, catalytically essential functional groups for the loop B domain are concentrated within an expanded minor groove, presenting a clear docking surface for the loop A domain.
Solution structure of the loop B domain from the hairpin ribozyme.,Butcher SE, Allain FH, Feigon J Nat Struct Biol. 1999 Mar;6(3):212-6. PMID:10074938[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Butcher SE, Allain FH, Feigon J. Solution structure of the loop B domain from the hairpin ribozyme. Nat Struct Biol. 1999 Mar;6(3):212-6. PMID:10074938 doi:10.1038/6651
