1nre

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1nre, 1 NMR models ()
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

RECEPTOR ASSOCIATED PROTEIN (RAP) DOMAIN 1, NMR, MINIMIZED AVERAGE STRUCTURE

Publication Abstract from PubMed

The three-dimensional structure of the N-terminal domain (residues 18-112) of alpha2-macroglobulin receptor-associated protein (RAP) has been determined by NMR spectroscopy. The structure consists of three helices composed of residues 23-34, 39-65, and 73-88. The three helices are arranged in an up-down-up antiparallel topology. The C-terminal 20 residues were shown not to be in a well defined conformation. A structural model for the binding of RAP to the family of low-density lipoprotein receptors is proposed. It defines a role in binding for both the unordered C terminus and the structural scaffold of the core structure. Pathogenic epitopes for the rat disease Heymann nephritis, an experimental model of human membranous glomerulonephritis, have been identified in RAP and in the large endocytic receptor gp330/megalin. Here we provide the three-dimensional structure of the pathogenic epitope in RAP. The amino acid residues known to form the epitope are in a helix-loop-helix conformation, and from the structure it is possible to rationalize the published results obtained from studies of fragments of the N-terminal domain.

The solution structure of the N-terminal domain of alpha2-macroglobulin receptor-associated protein., Nielsen PR, Ellgaard L, Etzerodt M, Thogersen HC, Poulsen FM, Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7521-5. PMID:9207124

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Disease

[AMRP_HUMAN] Note=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.

Function

[AMRP_HUMAN] Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.

About this Structure

1nre is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

  • Nielsen PR, Ellgaard L, Etzerodt M, Thogersen HC, Poulsen FM. The solution structure of the N-terminal domain of alpha2-macroglobulin receptor-associated protein. Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7521-5. PMID:9207124
  • Nurizzo D, Cutruzzola F, Arese M, Bourgeois D, Brunori M, Cambillau C, Tegoni M. Conformational changes occurring upon reduction and NO binding in nitrite reductase from Pseudomonas aeruginosa. Biochemistry. 1998 Oct 6;37(40):13987-96. PMID:9760233 doi:10.1021/bi981348y

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