2ait
From Proteopedia
DETERMINATION OF THE COMPLETE THREEDIMENSIONAL STRUCTURE OF THE ALPHAAMYLASE INHIBITOR TENDAMISTAT IN AQUEOUS SOLUTION BY NUCLEAR MAGNETIC RESONANCE AND DISTANCE GEOMETRY
Structural highlights
Function[IAA_STRTE] Inhibits mammalian alphaamylases specifically but has no action on plant and microbial alphaamylases. Forms a tight stoichiometric 1:1 complex with alphaamylase. Evolutionary ConservationCheck, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe complete threedimensional structure of the alphaamylase inhibitor Tendamistat in aqueous solution was determined by 1H nuclear magnetic resonance and distance geometry calculations using the program DISMAN. Compared to an earlier, preliminary determination of the polypeptide backbone conformation, stereospecific assignments were obtained for 41 of the 89 prochiral groups in the protein, and a much more extensive set of experimental constraints was collected, including 842 distance constraints from nuclear Overhauser effects and over 100 supplementary constraints from spinspin coupling constants and the identification of intramolecular hydrogen bonds. The complete protein molecule, including the amino acid sidechains is characterized by a group of nine structures corresponding to the results of the nine DISMAN calculations with minimal residual error functions. The average of the pairwise minimal rootmeansquare distances among these nine structures is 0.85 A for the polypeptide backbone, and 1.52 A for all the heavy atoms. The procedures used for the structure determination are described and a detailed analysis is presented of correlations between the experimental input data and the precision of the structure determination. Determination of the complete threedimensional structure of the alphaamylase inhibitor tendamistat in aqueous solution by nuclear magnetic resonance and distance geometry.,Kline AD, Braun W, Wuthrich K J Mol Biol. 1988 Dec 5;204(3):675724. PMID:3265733^{[1]} From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
