2k5b

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2k5b, 10 NMR models ()
Gene: HSP90AA1, HSP90A, HSPC1, HSPCA (Homo sapiens), CDC37, CDC37A (Homo sapiens)
Related: 1yes
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Human CDC37-HSP90 docking model based on NMR

Publication Abstract from PubMed

The cell division cycle protein 37 (Cdc37) and the 90-kDa heat shock protein (Hsp90) are molecular chaperones, which are crucial elements in the protein signaling pathway. The largest class of client proteins for Cdc37 and Hsp90 are protein kinases. The catalytic domains of these kinases are stabilized by Cdc37, and their proper folding and functioning is dependent on Hsp90. Here, we present the x-ray crystal structure of the 16-kDa middle domain of human Cdc37 at 1.88 angstroms resolution and the structure of this domain in complex with the 23-kDa N-terminal domain of human Hsp90 based on heteronuclear solution state NMR data and docking. Our results demonstrate that the middle domain of Cdc37 exists as a monomer. NMR and mutagenesis experiments reveal Leu-205 in Cdc37 as a key residue enabling complex formation. These findings can be very useful in the development of small molecule inhibitors against cancer.

The human Cdc37.Hsp90 complex studied by heteronuclear NMR spectroscopy., Sreeramulu S, Jonker HR, Langer T, Richter C, Lancaster CR, Schwalbe H, J Biol Chem. 2009 Feb 6;284(6):3885-96. Epub 2008 Dec 10. PMID:19073599

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2k5b is a 2 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA.

See Also

Reference

  • Sreeramulu S, Jonker HR, Langer T, Richter C, Lancaster CR, Schwalbe H. The human Cdc37.Hsp90 complex studied by heteronuclear NMR spectroscopy. J Biol Chem. 2009 Feb 6;284(6):3885-96. Epub 2008 Dec 10. PMID:19073599 doi:10.1074/jbc.M806715200

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