Structural highlights
Function
PXPB_BACSU Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate (PubMed:28830929). In addition, is a potent inhibitor of the autophosphorylation reaction of kinase A (kinA) and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. Is an inhibitor of the catalytic domain of kinase A affecting the ATP/ADP reactions and not the phosphotransferase functions of this domain. The inhibition is non-competitive with respect to ATP (PubMed:9334321).[1] [2]
References
- ↑ Niehaus TD, Elbadawi-Sidhu M, de Crecy-Lagard V, Fiehn O, Hanson AD. Discovery of a widespread prokaryotic 5-oxoprolinase that was hiding in plain sight. J Biol Chem. 2017 Sep 29;292(39):16360-16367. doi: 10.1074/jbc.M117.805028. Epub , 2017 Aug 22. PMID:28830929 doi:http://dx.doi.org/10.1074/jbc.M117.805028
- ↑ Wang L, Grau R, Perego M, Hoch JA. A novel histidine kinase inhibitor regulating development in Bacillus subtilis. Genes Dev. 1997 Oct 1;11(19):2569-79. doi: 10.1101/gad.11.19.2569. PMID:9334321 doi:http://dx.doi.org/10.1101/gad.11.19.2569
