2lom
From Proteopedia
Backbone structure of human membrane protein HIGD1A
Structural highlights
FunctionHIG1A_HUMAN Proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes.[1] Publication Abstract from PubMedAlthough nearly half of today's major pharmaceutical drugs target human integral membrane proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank, largely owing to inefficiencies in protein production. Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spectroscopy with systematically labeled proteins produced via cell-free expression. We report new backbone structures of six hIMPs, solved in only 18 months from 15 initial targets. Application of our protocols to an additional 135 hIMPs with molecular weight <30 kDa yielded 38 hIMPs suitable for structural characterization by solution NMR spectroscopy without additional optimization. Facile backbone structure determination of human membrane proteins by NMR spectroscopy.,Klammt C, Maslennikov I, Bayrhuber M, Eichmann C, Vajpai N, Chiu EJ, Blain KY, Esquivies L, Kwon JH, Balana B, Pieper U, Sali A, Slesinger PA, Kwiatkowski W, Riek R, Choe S Nat Methods. 2012 May 20;9(8):834-9. doi: 10.1038/nmeth.2033. PMID:22609626[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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