Enolase multiple sequence alignment
From Proteopedia
This page is a supplement to Introduction to Evolutionary Conservation. The portion of the multiple sequence alignment (MSA) below with a pink background is enlarged on that page.
Multiple Sequence Alignment (MSA) for enolase, an enzyme in glycolysis.
Key: IDENTICAL(*) SIMILAR(.) different
Consensus Percentage is set to 100%.
1 ENO_DROME|
Drosophila melanogaster --------mt IkaIkARqIy DSRGNPTVEv
DLtt-elglf rAavPSGAST 41
2 ENOA_HUMAN|
Homo sapiens ---------s IlkIhAReIf DSRGNPTVEv DLft-skglf
rAavPSGAST 40
3 ENO_METJA| Methanococcus janaschii
mlynmderfe IkdIvAReVi DSRGNPTVEv EVitkgngyg sAivPSGAST
50
4 ENO_ECOLI|
Escherichia coli --------sk IvkIiGReIi DSRGNPTVEa
EVhleggfvg mAaaPSGAST 42
5 ENO_BACSU|
Bacillus subtilis -------mpy IvdVyAReVl DSRGNPTVEv
EVytetgafg rAlvPSGAST 43
6 4ENL Yeast| Saccharomyces cerevisiae ---------a VskVyARsVy
DSRGNPTVEv ELtt-ekgvf rSivPSGAST 40
. . .* . *********
..
. ******
1 ENO_DROME| Drosophila melanogaster GvhEALElRD
nDKanYhGKS VlkAVghVNd tLgpelIkan ldvvdQasID
91
2 ENOA_HUMAN|
Homo sapiens GiyEALElRD nDKtrYmGKG
VskAVehINk tIapalVskk lnvteQekID 90
3 ENO_METJA| Methanococcus janaschii GthEALElRD
kEK-rFgGKG VlmAVenVNs iIrpeiLg-- ydarmQreID
97
4 ENO_ECOLI|
Escherichia coli GsrEALElRD gDKsrFlGKG
VtkAVaaVNg pIaqalIg-- kdakdQagID 90
5 ENO_BACSU| Bacillus
subtilis GeyEAVElRD gDKdrYlGKG VltAVnnVNe
iIapelLg-- fdvteQnaID 91
6 4ENL Yeast| Saccharomyces cerevisiae GvhEALEmRD
gDKskWmGKG VlhAVknVNd vIapafVkan idvsdQkaVD
90
* **.* ** .* . **. * ** .* . . * .*
1 ENO_DROME| Drosophila melanogaster nfmIklDGTe
NKSKfGANAI LGVSlAvaKA GAakkgvPLY
khIAdlagn- 140
2 ENOA_HUMAN|
Homo sapiens klmIemDGTe NKSKfGANAI LGVSlAvcKA
GAvekgvPLY rhIAdlagn- 139
3 ENO_METJA| Methanococcus janaschii timIelDGTp
NKSRlGANAI LAVSlAvaKA AAatakiPLY
kyLGgfn--- 144
4 ENO_ECOLI|
Escherichia coli kimIdlDGTe NKSKfGANAI LAVSlAnaKA
AAaakgmPLY ehIAelngt- 139
5 ENO_BACSU|
Bacillus subtilis qllIelDGTe NKGKlGANAI LGVSmAcaRA
AAdflqiPLY qyLGgfn--- 138
6 4ENL Yeast| Saccharomyces cerevisiae dflIslDGTa
NKSKlGANAI LGVSlAasRA AAaeknvPLY
khLAdlsksk 140
* *** **.. ***** *.** * .* .* *** .
1 ENO_DROME| Drosophila melanogaster -keiilPVPa
fNVINGGsHA gnkLamQEFM IlPtGAtsft
EAmKmGSeVY 189
2 ENOA_HUMAN|
Homo sapiens -sevilPVPa fNVINGGsHA gnkLamQEFM
IlPvGAanfr EAmRiGAeVY 188
3 ENO_METJA| Methanococcus janaschii --syvmPVPm
mNVINGGkHA gndLdlQEFM ImPvGAtsis
EAvRmGSeVY 192
4 ENO_ECOLI|
Escherichia coli pgkysmPVPm mNIINGGeHA dnnVdiQEFM
IqPvGAktvk EAiRmGSeVF 189
5 ENO_BACSU|
Bacillus subtilis --sktlPVPm mNIVNGGeHA
dnnVdiQEFM ImPvGApnfr EAlRmGAqIF 186
6 4ENL Yeast| Saccharomyces cerevisiae tspyvlPVPf
lNVLNGGsHA ggaLalQEFM IaPtGAktfa
EAlRiGSeVY 190
*** *..*** ** . **** * * ** ** . *. ..
1 ENO_DROME|
Drosophila melanogaster HhLknVikaK fgldataVGD EGGfAPnIqs
nkEALnlIsd AIakAGyt-- 237
2 ENOA_HUMAN|
Homo sapiens HnLknVikeK ygkdatnVGD EGGfAPnIle
nkEGLelLkt AIgkAGyt-- 236
3 ENO_METJA| Methanococcus janaschii HvLknVileK
ygknavnVGD EGGfAPpLkt srEALdlLte SVkkAGye--
240
4 ENO_ECOLI|
Escherichia coli HhLakVlkaK g--mntaVGD EGGyAPnLgs
naEALavIae AVkaAGyelg 237
5 ENO_BACSU|
Bacillus subtilis HsLksVlsaK g--lntaVGD EGGfAPnLgs
neEALqtIve AIekAGfkpg 234
6 4ENL Yeast| Saccharomyces cerevisiae HnLksLtkkR
ygasagnVGD EGGvAPnIqt aeEALdlIvd AIkaAG---- 238
** . . *** *** ** . *.* . .. **
1 ENO_DROME| Drosophila melanogaster gkIeiGmDvA
ASEFYk--dG qYdLdfknek sdksqwlpad klanlykefi 285
2 ENOA_HUMAN|
Homo sapiens dkVviGmDvA ASEFFr--sG kYdLdfksp-
ddpsryispd qladlyksfi 283
3 ENO_METJA| Methanococcus janaschii deVvfAlDaA
ASEFYk--dG yYyVegkk-- ------ltre elldyykalv 280
4 ENO_ECOLI|
Escherichia coli kdItlAmDcA ASEFYk--dG kYvLagegn-
----kaftse efthfleelt 280
5 ENO_BACSU|
Bacillus subtilis eeVklAmDaA SSEFYnkedG kYhLsgeg--
----vvktsa emvdwyeelv 278
6 4ENL Yeast| Saccharomyces cerevisiae gkVkiGlDcA
SSEFFk--dG kYdLdfknpn sdkskwltgp qladlyhslm 286
. . * * .***. * *
.
1 ENO_DROME| Drosophila melanogaster kdFPIVSIED
pfdqdhWeAw snltgctd-- IQIVGDDLtV TNpkrIatAV
333
2 ENOA_HUMAN|
Homo sapiens kdYPVVSIED pfdqddWgAw qkftasag-- IQVVGDDLtV
TNpkrIakAV 331
3 ENO_METJA| Methanococcus janaschii deYPIVSIED
pfheedFeGf amitkeld-- IQIVGDDLfV TNverLrkGI
328
4 ENO_ECOLI|
Escherichia coli kqYPIVSIED gldesdWdGf ayqtkvlgdk IQLVGDDLfV
TNtkiLkeGI 330
5 ENO_BACSU|
Bacillus subtilis skYPIISIED gldendWeGh kllterlgkk VQLVGDDLfV
TNtkkLseGI 328
6 4ENL Yeast| Saccharomyces cerevisiae krYPIVSIED
pfaeddWeAw shffktag-- IQIVADDLtV TNpkrIatAI
334
.*..**** . .
.*.*.*** * ** . ..
1 ENO_DROME| Drosophila melanogaster
ekkacncLLL KvNQIGtVtE siaAhllAkk nGWgtmVSHR
SGETEDsfIG 383
2 ENOA_HUMAN|
Homo sapiens nekscncLLL KvNQIGsVtE slqAcklAqa
nGWgvmVSHR SGETEDtfIA 381
3 ENO_METJA| Methanococcus janaschii
emkaanaLLL KvNQIGtLsE avdAaqlAfr nGYgvvVSHR
SGETEDttIA 378
4 ENO_ECOLI|
Escherichia coli ekgiansILI KfNQIGsLtE tlaAikmAkd
aGYtavISHR SGETEDatIA 380
5 ENO_BACSU|
Bacillus subtilis kngvgnsILI KvNQIGtLtE tfdAiemAkr
aGYtavISHR SGETEDstIA 378
6 4ENL Yeast| Saccharomyces cerevisiae ekkaadaLLL
KvNQIGtLsE sikAaqdSfa aGWgvmVSHR SGETEDtfIA
384
.*. . **** . *
* . *. .*** ****** *.
1 ENO_DROME| Drosophila melanogaster DLvVGlstGQ
IKTGApcRsE RlAKyNQILR IEeeIGagvk
FaGksfgkpq 433
2 ENOA_HUMAN|
Homo sapiens DLvVGlctGQ IKTGApcRsE RlAKyNQLLR
IEeeLGskak FaGrnfrnpl 431
3 ENO_METJA| Methanococcus janaschii DLsVAlnsGQ
IKTGApaRgE RtAKyNQLIR IEqeLG-lsk
YaGrnfrcpf 427
4 ENO_ECOLI|
Escherichia coli DLaVGtaaGQ IKTGSmsRsD
RvAKyNQLIR IEeaLGekap YnGrkeikgq 430
5 ENO_BACSU|
Bacillus subtilis DIaVAtnaGQ IKTGApsRtD
RvAKyNQLLR IEdqLAetaq YhGinsfynl 428
6 4ENL Yeast| Saccharomyces cerevisiae DLvVGlrtGQ
IKTGApaRsE RlAKlNQLLR IEeeLGdnav
FaGenfhhgd 434
*. *. ** ****. * . * ** **..* ** .. . *
1 ENO_DROME|
Drosophila melanogaster -- 433
2 ENOA_HUMAN|
Homo sapiens ak 433
3 ENO_METJA| Methanococcus janaschii -- 427
4 ENO_ECOLI|
Escherichia coli a- 431
5 ENO_BACSU|
Bacillus subtilis nk 430
6 4ENL Yeast| Saccharomyces cerevisiae kl 436
Summary of categories applied to a total of 436 residues in the PDB file sequence for a consensus level setting of 100%:
33% (142) IDENTICAL(*) 16% (68) SIMILAR(.) 52% (226) different
Definitions of SIMILAR groups of amino acids:ILV Ile Leu Val hydrophobic, aliphatic / medium-sized side-chain FWY Phe Trp Tyr hydrophobic, aromatic / large side-chain (Tyr polar) KRH Lys Arg His + charge DE Asp Glu - charge GAS Gly Ala Ser small P Pro helix breaking C Cys disulfide-forming TNQM Thr Asn Gln Met polar (except Met)