Glutamine synthetase
From Proteopedia
FunctionGlutamine synthetase (GS) is an essential enzyme in the cellular nitrogen metabolism and has been found to play a role in both ammonia assimilation and glutamine byosynthesis.[1] It is the only enzyme capable of glutamine synthesis and is required for the two step process synthesizing glutamate to glutamine. The glutamine produced is an essential precursor for purine and pyrimidine synthesis, a modulator of protein turnover or an intermediate for gluconeogenesis and acid-base balance. [2] StructureThe structure of glutamine synthetase is made of two layers, each containing 6 subunits, for a total of 12 subunits. [3] Each subunit contains an active site which is defined by a cylindrical shape formed by six antiparalel β starnds contributed by one subunit and two more strands by the neighbouring subunit. [3] In each cylindrical active site there are two Mn2+ ions: Mn 2+ 469 and 470. Both are attached to three protein chains and two water molecules, one of the water molecules are shared by both Mn2+. The protein ligands. Attached to Mn 2+ 469 is: Glu-131, GLu-212 and Glu-220, and the protein ligands attached to Mn 2+ are: Glu-129, His-269 and Glu-357.[3] Unique to glutamine synthetase, unlike other enzymes, is it's "passive site". This refers to the central loop which is formed by a segment of the backbone that extends into the central aqueous cavity. [3]Unlike other sites in glutamine synthetase under the same conditions, this site is suceptible to proteolysis by four secreated proteases from the V8 protease of Staphylococcus aureus.[3] Additional details in ClassesGlutamine synthetase has been described as having three destinctive types:[4]
3D structures of glutamine synthetaseGlutamine synthetase 3D structures
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References
- ↑ 1.0 1.1 1.2 Kumada Y, Benson DR, Hillemann D, Hosted TJ, Rochefort DA, Thompson CJ, Wohlleben W, Tateno Y. Evolution of the glutamine synthetase gene, one of the oldest existing and functioning genes. Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):3009-13. PMID:8096645
- ↑ He,Youji, Hakvoort,Theodorus, B.M., Kohler,S.Eleonor, Vermeulen,Jacqueline L.M.,Rudi de Waart, D., Theije,Chiel de, Gabrie A.M. ten Have, Van Eijk,Hans M.H., Kunne,Cindy, Labruyere,Wilelmina T., Houten,Sander M., Sokolovic,Mika, Tuijter,Jan M., Deutz,Nicolaas E.P., and Lamers, Wouter H. Glutamine Synthetase in muscle is required for glutamine production druing fasting and extrahepatic ammonia detoxification. The American Society for Biochemistry and Molecular Biology, January 11, 2010
- ↑ 3.0 3.1 3.2 3.3 3.4 Yamashita MM, Almassy RJ, Janson CA, Cascio D, Eisenberg D. Refined atomic model of glutamine synthetase at 3.5 A resolution. J Biol Chem. 1989 Oct 25;264(30):17681-90. PMID:2572586
- ↑ 4.0 4.1 4.2 Brown JR, Masuchi Y, Robb FT, Doolittle WF. Evolutionary relationships of bacterial and archaeal glutamine synthetase genes. J Mol Evol. 1994 Jun;38(6):566-76. PMID:7916055
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