Heidi Hu/Sandbox 2

From Proteopedia

Jump to: navigation, search

One of the CBI Molecules being studied in the University of Massachusetts Amherst Chemistry-Biology Interface Program at UMass Amherst and on display at the Molecular Playground.

Structures of HypA (PDB ID: 2KDX), (PDB ID: 3A43), and (PDB ID: 3A44)

Drag the structure with the mouse to rotate


Contents

Introduction

Helicobacter pylori is a pathogenic bacterium that colonizes the human gastric mucosa, which can cause peptic ulcers and has been linked to stomach cancers.[1] H. pylori requires the activity of nickel-dependent enzymes, [NiFe]-Hydrogenase (H2ase) and urease, to survive in the acidic environment of the stomach.[2][3][4] Thus nickel is an important nutrient for H. pylori.


Accessory proteins HypABCDEF and UreIEFGH facilitate the maturation of [NiFe]-H2ase and urease respectively[5]. HypA is a nickel metallochaperone normally associated with the maturation of [NiFe]-H2ase[6]. In H. pylori, however, it is also require for the full activy of urease, despite the presence of the urease-specific Ni-chaperone, UreE[7].


HypA


The H. pyloriHypA (HpHypA) is a 13.2kDa Ni-chaperone with both a nickel binding site and and structural zinc site. Zn(II) is coordinated by two CXXC motif each with a flanking histidine, whereas the Ni(II) is known to bind to the N-terminus MHE motif[8]. The HpHypA protein has also been characterized as a monomer and a homodimer. The (N-terminal tag colored in red) has been solved by NMR (PDB ID: 2KDX)[9]. The monomeric structure shows an containing the N- and C-termini well separated from the . The homodimeric HpHypA has been characterized by NMR to have the similar overall structure with discrepancies to the metal binding sites[8]. The metal sites in HpHypA have been characterized by X-ray absorption spectroscopy (XAS) at pH 6.3 and 7.2 to similate the internal pH of H. pylori under acid shock or at neutral pH conditions[8] respectively. While the Ni(II) site is 6-coordinate N/O under both pH conditions, the Zn(II) coordination changes from Cys4 at neutral pH to Cys2His2 at acidic pH with nickel-bound[8] (Fig. 1). Changes to the coordination of structural Zinc site in response to pH has been hypothesized to be linked to changes in HypA conformations and protein interaction partners[8].

Fig. 1: Diagram of Ni- and pH-dependent structural changes to Zn(II) site of HpHypA (adapted figure)
Fig. 1: Diagram of Ni- and pH-dependent structural changes to Zn(II) site of HpHypA (adapted figure[8])

The crystal structure of (PDB ID: 3A43) and (PDB ID: 3A44) HypA from Thermococcus kodakarensis has been solved[10]. The HypA homodimer from T. kodakarensis shows a switch dimer, where one beta strand of each alpha/beta lobe comes from the opposite subunit. Additionally, each is coordinated by CXXC motifs from a different subunit. These observations were consistent with the H. pylori homodimeric HypA NMR data[8].

Research Interests

The Ni- and pH-dependent changes in the H. pylori HypA structural zinc site suggests multiple conformations of this protein. Thus HypA is likely interfacing between the maturation of [NiFe]-H2ase and/or urease in H. pylori in a pH-dependent manner, as it is required for the full activation of both of these nickel enzymes. The Maroney Lab at the University of Massachusetts Amherst is interested in characterizing the conformational changes in HypA and its interaction partners under neutral and acid shock conditions.


References

  1. ↑ Kuipers EJ. Helicobacter pylori and the risk and management of associated diseases: gastritis, ulcer disease, atrophic gastritis and gastric cancer. Aliment Pharmacol Ther. 1997 Apr;11 Suppl 1:71-88. PMID:9146793
  2. ↑ Eaton KA, Brooks CL, Morgan DR, Krakowka S. Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets. Infect Immun. 1991 Jul;59(7):2470-5. PMID:2050411
  3. ↑ Eaton KA, Krakowka S. Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori. Infect Immun. 1994 Sep;62(9):3604-7. PMID:8063376
  4. ↑ Olson JW, Maier RJ. Molecular hydrogen as an energy source for Helicobacter pylori. Science. 2002 Nov 29;298(5599):1788-90. PMID:12459589 doi:10.1126/science.1077123
  5. ↑ Tomb JF, White O, Kerlavage AR, Clayton RA, Sutton GG, Fleischmann RD, Ketchum KA, Klenk HP, Gill S, Dougherty BA, Nelson K, Quackenbush J, Zhou L, Kirkness EF, Peterson S, Loftus B, Richardson D, Dodson R, Khalak HG, Glodek A, McKenney K, Fitzegerald LM, Lee N, Adams MD, Hickey EK, Berg DE, Gocayne JD, Utterback TR, Peterson JD, Kelley JM, Cotton MD, Weidman JM, Fujii C, Bowman C, Watthey L, Wallin E, Hayes WS, Borodovsky M, Karp PD, Smith HO, Fraser CM, Venter JC. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature. 1997 Aug 7;388(6642):539-47. PMID:9252185 doi:10.1038/41483
  6. ↑ Higgins KA, Carr CE, Maroney MJ. Specific metal recognition in nickel trafficking. Biochemistry. 2012 Oct 9;51(40):7816-32. doi: 10.1021/bi300981m. Epub 2012 Sep, 28. PMID:22970729 doi:10.1021/bi300981m
  7. ↑ Olson JW, Mehta NS, Maier RJ. Requirement of nickel metabolism proteins HypA and HypB for full activity of both hydrogenase and urease in Helicobacter pylori. Mol Microbiol. 2001 Jan;39(1):176-82. PMID:11123699
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 8.6 Herbst RW, Perovic I, Martin-Diaconescu V, O'Brien K, Chivers PT, Pochapsky SS, Pochapsky TC, Maroney MJ. Communication between the zinc and nickel sites in dimeric HypA: metal recognition and pH sensing. J Am Chem Soc. 2010 Aug 4;132(30):10338-51. doi: 10.1021/ja1005724. PMID:20662514 doi:10.1021/ja1005724
  9. ↑ Xia W, Li H, Sze KH, Sun H. Structure of a nickel chaperone, HypA, from Helicobacter pylori reveals two distinct metal binding sites. J Am Chem Soc. 2009 Jul 29;131(29):10031-40. PMID:19621959 doi:10.1021/ja900543y
  10. ↑ Watanabe S, Arai T, Matsumi R, Atomi H, Imanaka T, Miki K. Crystal structure of HypA, a nickel-binding metallochaperone for [NiFe] hydrogenase maturation. J Mol Biol. 2009 Dec 4;394(3):448-59. Epub 2009 Sep 19. PMID:19769985 doi:10.1016/j.jmb.2009.09.030


Also See

2kdx
3a43
3a44

Proteopedia Page Contributors and Editors (what is this?)

Heidi Hu

Retrieved from "http://proteopedia.org/wiki/index.php/Heidi_Hu/Sandbox_2"
Personal tools