Resolvase

From Proteopedia

Jump to: navigation, search

Function

Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.

  • Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands[1].
  • Tyr-recombinases include Cre which recombines loxP sites, FLP and lambda integrase (LamInt).
  • Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.
  • RadA recombinase promotes DNA recombination. Detailed analysis of its dimeric structure suggests mechanisms for junction isomerization and communication between the two active sites [2].
  • RadB recombinase is a paralogue of RadA assisting in RadA-mediated strand exchange [3].
  • Hin-recombinase (HRec) is a protein of Salmonella. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions[4].
  • Tre recombinase recognizes a target sequence of the HIV virus resulting in excision and eradication of the provirus from infected human cells [5].

3D structures of resolvase

Resolvase 3D structures


Yeast Holliday junction resolvase dimer complex with sulfate, 1kcf

Drag the structure with the mouse to rotate

References

  1. ↑ West SC. The search for a human Holliday junction resolvase. Biochem Soc Trans. 2009 Jun;37(Pt 3):519-26. doi: 10.1042/BST0370519. PMID:19442245 doi:http://dx.doi.org/10.1042/BST0370519
  2. ↑ Haldenby S, White MF, Allers T. RecA family proteins in archaea: RadA and its cousins. Biochem Soc Trans. 2009 Feb;37(Pt 1):102-7. doi: 10.1042/BST0370102. PMID:19143611 doi:http://dx.doi.org/10.1042/BST0370102
  3. ↑ Wardell K, Haldenby S, Jones N, Liddell S, Ngo GHP, Allers T. RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator. DNA Repair (Amst). 2017 Jul;55:7-16. PMID:28501701 doi:10.1016/j.dnarep.2017.04.005
  4. ↑ Dhar G, Sanders ER, Johnson RC. Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands. Cell. 2004 Oct 1;119(1):33-45. PMID:15454079 doi:http://dx.doi.org/10.1016/j.cell.2004.09.010
  5. ↑ Buchholz F. Molecular evolution of the tre recombinase. J Vis Exp. 2008 May 29;(15):791. PMID:19066582 doi:10.3791/791

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman, Jaime Prilusky

Retrieved from "http://proteopedia.org/wiki/index.php/Resolvase"
Personal tools