Ribozyme

From Proteopedia

Ribozyme (RZ) is an RNA molecule which catalyzes a chemical reaction[1]. Glucosamine-6-phosphate activated RZ (glmS RZ) is a RZ and a riboswitch which regulates genes in response to metabolites. Leadzyme is a small, artificially-made RZ which can cleave RNA in the presence of lead. For more details on leadzyme see Student Projects for UMass Chemistry 423 Spring 2012-10. Hairpin RZ and hammerhead RZ are named based on their shape[2]. Group I intron catalyzes its own selfish splicing[3]. Comparative genomic analysis revealed Twister, Twister sister, Hatchet, and Pistol [4][5]. Articles in Proteopedia concerning ribozymes include: Azoarcus group I intron Kink-turn motif Ribosome The Large Ribosomal Subunit Hairpin Ribozyme Human CPEB3 ribozyme's P4 domain To view automatically PDB-IDs concerning ribozymes, see: Ribozyme Catalytic RNA GlmS Hammerhead Hairpin Hepatitis delta virus Twister Twister sister Hatchet Pistol Group I intron Varkud satellite

spinqualitylabelsall models Hammerhead ribozyme, (PDB-ID 379d) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of Ribozymes

Ribozyme 3D structures

References

1. ↑ Tang J, Breaker RR. Rational design of allosteric ribozymes. Chem Biol. 1997 Jun;4(6):453-9. PMID:9224568
2. ↑ Amarzguioui M, Prydz H. Hammerhead ribozyme design and application. Cell Mol Life Sci. 1998 Nov;54(11):1175-202. PMID:9849614 doi:http://dx.doi.org/10.1007/s000180050247
3. ↑ Cech TR. Self-splicing of group I introns. Annu Rev Biochem. 1990;59:543-68. PMID:2197983 doi:http://dx.doi.org/10.1146/annurev.bi.59.070190.002551
4. ↑ Roth A, Weinberg Z, Chen AG, Kim PB, Ames TD, Breaker RR. A widespread self-cleaving ribozyme class is revealed by bioinformatics. Nat Chem Biol. 2014 Jan;10(1):56-60. doi: 10.1038/nchembio.1386. Epub 2013 Nov, 17. PMID:24240507 doi:http://dx.doi.org/10.1038/nchembio.1386
5. ↑ Weinberg Z, Kim PB, Chen TH, Li S, Harris KA, Lunse CE, Breaker RR. New classes of self-cleaving ribozymes revealed by comparative genomics analysis. Nat Chem Biol. 2015 Aug;11(8):606-10. doi: 10.1038/nchembio.1846. Epub 2015 Jul, 13. PMID:26167874 doi:http://dx.doi.org/10.1038/nchembio.1846