12iy
Locally refined cryo-EM structure of human cannabinoid receptor 2 with agonist '5249
Structural highlights
FunctionCNR2_HUMAN Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.[1] [2] [3] [4] Publication Abstract from PubMedCannabinoid receptors are both therapeutically attractive and are interesting model systems for structure-based methods. Here we investigated topical questions in library docking using the CB2 receptor. While a CB1R docking campaign found potent but nonselective ligands, here subtype selective ligands were found by targeting polar residues. Hit rates and hit affinities improved with library size, but docking against active and inactive receptor states did not reliably bias toward agonists or antagonists. Cryo-EM structures of two of the new agonists superposed well on the docking predictions. Structure-based optimization led to 10- to 140-fold improvements within three series, consistent with well-behaved ligands. Hit rates with an explicit 2.6 billion molecule library resembled those of an implied 11 billion molecule library from a building-block method, supporting the latter's ability to explore this space, though higher affinities were discovered from the explicit set. Implications for future studies are considered. Library Docking for Cannabinoid-2 Receptor Ligands.,Rachman MM, Iliopoulos-Tsoutsouvas C, Sacco MD, Xu X, Wu CG, Santos E, Glenn IS, Paris L, Cahill MK, Ganapathy S, Tummino TA, Moroz YS, Radchenko DS, Okorie M, Tawfik VL, Irwin JJ, Makriyannis A, Skiniotis G, Shoichet BK J Med Chem. 2026 Jul 3. doi: 10.1021/acs.jmedchem.6c00835. PMID:42397716[5] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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