1k9o

CRYSTAL STRUCTURE OF MICHAELIS SERPIN-TRYPSIN COMPLEX

spinqualitylabelsall models 1k9o, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1k9o is a 2 chain structure with sequence from Manduca sexta and Rattus norvegicus. This structure supersedes the now removed PDB entry 1i99. The May 2004 RCSB PDB Molecule of the Month feature on Serpins by David S. Goodsell is 10.2210/rcsb_pdb/mom_2004_5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SERA_MANSE Inhibits elastase. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Serine protease inhibitors (serpins) regulate the activities of circulating proteases. Serpins inhibit proteases by acylating the serine hydroxyl at their active sites. Before deacylation and complete proteolysis of the serpin can occur, massive conformational changes are triggered in the serpin while maintaining the covalent linkage between the protease and serpin. Here we report the structure of a serpin-trypsin Michaelis complex, which we visualized by using the S195A trypsin mutant to prevent covalent complex formation. This encounter complex reveals a more extensive interaction surface than that present in small inhibitor-protease complexes and is a template for modeling other serpin-protease pairs. Mutations of several serpin residues at the interface reduced the inhibitory activity of the serpin. The serine residue C-terminal to the scissile peptide bond is found in a closer than usual interaction with His 57 at the active site of trypsin. The structure of a Michaelis serpin-protease complex.,Ye S, Cech AL, Belmares R, Bergstrom RC, Tong Y, Corey DR, Kanost MR, Goldsmith EJ Nat Struct Biol. 2001 Nov;8(11):979-83. PMID:11685246 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Serpin 3D structures Trypsin 3D structures

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