1nx4 is a 3 chain structure with sequence from Pectobacterium carotovorum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
CARC_PECCC Catalyzes the Fe(2+) and alpha-ketoglutarate-dependent conversion of (3S,5S)-carbapenam to (5R)-carbapenem, an essential step in carbapenem antibiotic biosynthesis.[1][2][3][4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
↑McGowan SJ, Sebaihia M, O'Leary S, Hardie KR, Williams P, Stewart GS, Bycroft BW, Salmond GP. Analysis of the carbapenem gene cluster of Erwinia carotovora: definition of the antibiotic biosynthetic genes and evidence for a novel beta-lactam resistance mechanism. Mol Microbiol. 1997 Nov;26(3):545-56. PMID:9402024
↑Stapon A, Li R, Townsend CA. Carbapenem biosynthesis: confirmation of stereochemical assignments and the role of CarC in the ring stereoinversion process from L-proline. J Am Chem Soc. 2003 Jul 16;125(28):8486-93. PMID:12848554 doi:https://dx.doi.org/10.1021/ja034248a
↑Sleeman MC, Smith P, Kellam B, Chhabra SR, Bycroft BW, Schofield CJ. Biosynthesis of carbapenem antibiotics: new carbapenam substrates for carbapenem synthase (CarC). Chembiochem. 2004 Jun 7;5(6):879-82. PMID:15174175 doi:https://dx.doi.org/10.1002/cbic.200300908
↑Clifton IJ, Doan LX, Sleeman MC, Topf M, Suzuki H, Wilmouth RC, Schofield CJ. Crystal structure of carbapenem synthase (CarC). J Biol Chem. 2003 Jun 6;278(23):20843-50. Epub 2003 Feb 28. PMID:12611886 doi:10.1074/jbc.M213054200
