1oln

Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA

spinqualitylabelsall models 1oln Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1oln is a 3 chain structure with sequence from Streptomyces azureus and Thermotoga maritima. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Hybrid , Solution NMR , Theoretical Model, 1 model Ligands: BB9, DBU, DCY, DHA, MH6, NH2, QUA, TS9 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RL11_THEMA This protein binds directly to 23S ribosomal RNA. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Thiostrepton and micrococcin inhibit protein synthesis by binding to the L11 binding domain (L11BD) of 23S ribosomal RNA. The two compounds are structurally related, yet they produce different effects on ribosomal RNA in footprinting experiments and on elongation factor-G (EF-G)-dependent GTP hydrolysis. Using NMR and an assay based on A1067 methylation by thiostrepton-resistance methyltransferase, we show that the related thiazoles, nosiheptide and siomycin, also bind to this region. The effect of all four antibiotics on EF-G-dependent GTP hydrolysis and EF-G-GDP-ribosome complex formation was studied. Our NMR and biochemical data demonstrate that thiostrepton, nosiheptide, and siomycin share a common profile, which differs from that of micrococcin. We have generated a three-dimensional (3D) model for the interaction of thiostrepton with L11BD RNA. The model rationalizes the differences between micrococcin and the thiostrepton-like antibiotics interacting with L11BD. Structural basis for contrasting activities of ribosome binding thiazole antibiotics.,Lentzen G, Klinck R, Matassova N, Aboul-ela F, Murchie AI Chem Biol. 2003 Aug;10(8):769-78. PMID:12954336 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosomal protein L11 3D structures

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