1qk7

Solution structure of Selenocosmia huwena lectin-I(SHL-I) by 2D-NMR

spinqualitylabelsall models 1qk7 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1qk7 is a 1 chain structure with sequence from Cyriopagopus schmidti. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 20 models Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TXLA1_CYRSC Agglutinates human and mice erythrocytes. This activity can be specifically inhibited by mannosamine. This lectin shows very low toxicity in both mammals and insects. Publication Abstract from PubMed The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex. Three-dimensional structure of Selenocosmia huwena lectin-I (SHL-I) from the venom of the spider Selenocosmia huwena by 2D-NMR.,Lu S, Liang S, Gu X J Protein Chem. 1999 Jul;18(5):609-17. PMID:10524779 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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