1t0k

Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex

spinqualitylabelsall models 1t0k, resolution 3.24Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1t0k is a 4 chain structure with sequence from Escherichia coli and Saccharomyces cerevisiae. This structure supersedes the now removed PDB entries 1ck5, 1ck8, 1cn8 and 1cn9. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.24Å Ligands: GLC, PRD_900010 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RL30_YEAST Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed L30e, a Saccharomyces cervisiae ribosomal protein, regulates its own expression by binding to a purine-rich asymmetric internal loop located in both its pre-mRNA and mature mRNA. A crystal structure of an MBP-L30e fusion protein in complex with an RNA containing the pre-mRNA regulatory site was solved at 3.24 A. Interestingly, the structure of the RNA differed from that observed in a previously determined NMR structure of the complex. Analysis of the NMR data led to the identification of a single imino proton resonance in the internal loop that had been incorrectly assigned and was principally responsible for the erroneous RNA structure. A structure refinement was performed using both the X-ray diffraction data and the NMR-derived distance and angle restraints. The joint NMR and X-ray refinement resulted in improved stereochemistry and lower crystallographic R factors. The RNA internal loop of the MBP-L30e-mRNA complex adopts the canonical K-turn fold. Joint X-ray and NMR refinement of the yeast L30e-mRNA complex.,Chao JA, Williamson JR Structure. 2004 Jul;12(7):1165-76. PMID:15242593 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Kink-turn motif Maltose-binding protein 3D structures Ribosomal protein L30 User:Wayne Decatur/kink-turn motif

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