1uon

REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION

Loading... Status: waiting for Mol* initialization Structural highlights 1uon is a 3 chain structure with sequence from Reovirus sp. and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 7.6Å Ligands: CH1, MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8V1A3_9REOV Publication Abstract from PubMed Reovirus is an icosahedral, double-stranded (ds) RNA virus that uses viral polymerases packaged within the viral core to transcribe its ten distinct plus-strand RNAs. To localize these polymerases, the structure of the reovirion was refined to a resolution of 7.6 A by cryo-electron microscopy (cryo-EM) and three-dimensional (3D) image reconstruction. X-ray crystal models of reovirus proteins, including polymerase lambda 3, were then fitted into the density map. Each copy of lambda 3 was found anchored to the inner surface of the icosahedral core shell, making major contacts with three molecules of shell protein lambda 1 and overlapping, but not centering on, a five-fold axis. The overlap explains why only one copy of lambda 3 is bound per vertex. lambda 3 is furthermore oriented with its transcript exit channel facing a small channel through the lambda 1 shell, suggesting how the nascent RNA is passed into the large external cavity of the pentameric capping enzyme complex formed by protein lambda 2. Reovirus polymerase lambda 3 localized by cryo-electron microscopy of virions at a resolution of 7.6 A.,Zhang X, Walker SB, Chipman PR, Nibert ML, Baker TS Nat Struct Biol. 2003 Dec;10(12):1011-8. Epub 2003 Nov 9. PMID:14608373 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Virus coat proteins 3D structures

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