1w5t

Structure of the Aeropyrum Pernix ORC2 protein (ADPNP-ADP complexes)

spinqualitylabelsall models 1w5t, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1w5t is a 3 chain structure with sequence from Aeropyrum pernix. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Ligands: ADP, ANP, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CDC62_AERPE Involved in regulation of DNA replication (By similarity). Binds DNA.[HAMAP-Rule:MF_01407] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Archaea contain one or more proteins with homology to eukaryotic ORC/Cdc6 proteins. Sequence analysis suggests the existence of at least two subfamilies of these proteins, for which we propose the nomenclature ORC1 and ORC2. We have determined crystal structures of the ORC2 protein from the archaeon Aeropyrum pernix in complexes with ADP or a non-hydrolysable ATP analogue, ADPNP. Between two crystal forms, there are three crystallographically independent views of the ADP complex and two of the ADPNP complex. The protein molecules in the three complexes with ADP adopt very different conformations, while the two complexes with ADPNP are the same. These structures indicate that there is considerable conformational flexibility in ORC2 but that ATP binding stabilises a single conformation. We show that the ORC2 protein can bind DNA, and that this activity is associated with the C-terminal domain of the protein. We present a model for the interaction of the winged helix (WH) domain of ORC2 with DNA that differs from that proposed previously for Pyrobaculum aerophilum ORC/Cdc6. Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix.,Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB J Mol Biol. 2004 Oct 22;343(3):547-57. PMID:15465044 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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