Crystal structure of the C123S 2-methylisocitrate lyase mutant from Escherichia coli in complex with the reaction product, Mg(II)-pyruvate and succinate
1xg3 is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
PRPB_ECOLI Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate.[1][2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
↑Brock M, Darley D, Textor S, Buckel W. 2-Methylisocitrate lyases from the bacterium Escherichia coli and the filamentous fungus Aspergillus nidulans: characterization and comparison of both enzymes. Eur J Biochem. 2001 Jun;268(12):3577-86. PMID:11422389
↑Liu S, Lu Z, Han Y, Melamud E, Dunaway-Mariano D, Herzberg O. Crystal structures of 2-methylisocitrate lyase in complex with product and with isocitrate inhibitor provide insight into lyase substrate specificity, catalysis and evolution. Biochemistry. 2005 Mar 1;44(8):2949-62. PMID:15723538 doi:https://dx.doi.org/10.1021/bi0479712
