1zhs

Crystal structure of MVL bound to Man3GlcNAc2

spinqualitylabelsall models 1zhs, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1zhs is a 8 chain structure with sequence from Microcystis viridis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: BMA, EDO, MAN, NAG, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function MVL_MICVR Carbohydrate-binding protein that binds oligomannosides such as Man(6)GlcNAc(2) with sub-micromolar affinities. The specificity of MVL is unique in that its minimal target comprises the Man-alpha-(1->6)-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc tetrasaccharide core (Man(2)A) found in N-linked oligomannosides. Displays hemagglutininating activity on rabbit, horse and hen erythrocytes. This activity is inhibited by yeast mannan. Does not bind mono- and disachharides. Inhibits HIV-1 envelope-mediated cell fusion at nanomolar concentrations through carbohydrate-mediated interactions with high-mannose residues on the surface of the HIV envelope glycoprotein gp120. Unexpectedly for a lectin, one of the 2 oligomannose binding sites of MVL can catalyze the cleavage of chitin fragments (such as chitotriose, i.e. GlcNAc(3) or GlcNAc-beta-(1->4)-GlcNAcbeta-(1->4)-GlcNAc, and chitotetraose, i.e. GlcNAc(4)) to GlcNAc. This weak glycosidase activity is restricted to the C-terminal carbohydrate-binding site. Does not cleave Man(3)GlcNAc(2) or the tetrasaccharide Man(2)A. See Also Mannose-binding protein

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