22tu

Human 80S ribosome in complex with DHX29

spinqualitylabelsall models 22tu, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 22tu is a 11 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3Å Ligands: MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS30_HUMAN Publication Abstract from PubMed Synonymous codon usage controls global gene expression in both prokaryotic and eukaryotic species. Nonoptimal codons are known to induce mRNA decay; however, the underlying molecular mechanism remains poorly understood in human cells. Through genome-wide CRISPR screening, we identified the RNA-binding protein DHX29 as a critical regulator of codon-dependent gene expression. Cryogenic electron microscopy and selective ribosome profiling demonstrated that DHX29 directly interacts with the A-site entrance of the translating 80S ribosome, the binding site for the eEF1A*GTP*aminoacyl-tRNA ternary complex, suggesting a role in monitoring aminoacyl-tRNA sampling. Proteomic analysis further revealed that DHX29 recruits the GIGYF2*4EHP complex to mediate global suppression of nonoptimal mRNAs. These findings establish a mechanistic link between synonymous codon usage and the regulation of gene expression. Human DHX29 detects nonoptimal codon usage to regulate mRNA stability.,Hia F, Wu Y, Yoshinaga M, Goto-Ito S, Iwasaki W, Imami K, Toh H, Han P, Cai T, Ohira T, Fukao A, Standley DM, Shichino Y, Takegawa M, Fujiwara T, Suzuki T, Iwasaki S, Bassik MC, Ito T, Takeuchi O Science. 2026 Mar 19:eadw0288. doi: 10.1126/science.adw0288. PMID:41855277 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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