23vj

Crystal structure of the MafR protein from Enterococcus faecalis

spinqualitylabelsall models 23vj, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 23vj is a 2 chain structure with sequence from Enterococcus faecalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Phosphotransferase system regulatory domain-containing virulence regulators (PCVRs), including Enterococcus faecalis MafR and Streptococcus pneumoniae Mga-like protein (MgaSpn), are transcription factors in pathogenic bacteria that control the transcription of virulence genes by binding dsDNA in promoter regions. Although PCVRs play critical roles in bacterial pathogenesis, the structural and molecular basis by which PCVRs recognize dsDNA for transcriptional regulation has remained unclear. Here, we present the unique structure of MafR and demonstrate the critical role of its two N-terminal helix-turn-helix (HTH) domains in dsDNA binding. MafR adopts a five-domain architecture with an open-ring conformation and dimerizes into a table-like structure, in which the C-terminal domain mediates dimerization in the middle of the dimer structure, and the two N-terminal HTH domains project from one face of the dimer. Our extensive modeling, biochemical, and mutational analyses of the MgaSpn-dsDNA interaction reveal that the tandem HTH domains are required for dsDNA binding by inserting their recognition helices into the major groove of dsDNA. Our findings highlight a unique mode of DNA recognition by PCVRs, in which two HTH domains are simultaneously employed for DNA binding, rather than the single HTH domain typically used by conventional HTH-containing transcriptional regulators. Unique structural features and DNA recognition mechanisms of the PRD-containing virulence regulators MafR and MgaSpn.,Park MA, Ahn SY, Cho SY, Yoon SI Biochem Biophys Res Commun. 2026 May 21;814:153625. doi: , 10.1016/j.bbrc.2026.153625. Epub 2026 Mar 17. PMID:41865467 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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