24ne

Crystal Structure of Cypridina luciferase

spinqualitylabelsall models 24ne, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 24ne is a 1 chain structure with sequence from Cypridina noctiluca. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3Å Ligands: NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Bioluminescence occurs in marine organisms and involves the oxidation of imidazopyrazinone-type luciferin catalyzed by luciferase. Although chemiluminescence mechanisms have been studied using luciferin analogs in aprotic solvents, the enzymatic reaction within luciferase remains poorly understood due to the lack of structural information. Cypridina luciferin (CypL), used by Vargula hilgendorfii, is an imidazopyrazinone compound known for its high quantum yield and turnover rate. Here, we report the crystal structure of Cypridina luciferase (CLuc) and its reaction product, Cypridina oxyluciferin (CypOL). Structural analysis and mutagenesis suggest that the amino acid residue H542 may mediate either deprotonation at the N7 position or stabilization of the anionic form of CypL in the first step of the oxidation process. This mechanism, together with strict substrate recognition, would provide a clue to investigate the molecular basis of CLuc's efficient light emission and advance understanding of imidazopyrazinone-type bioluminescence. Three-dimensional structure of Cypridina luciferase illuminates the mechanism of bioluminescence of imidazopyrazinone-type luciferin.,Kihira K, Yasuno R, Kanie S, Wu C, Mitani Y, Ohmiya Y Int J Biol Macromol. 2026 Mar 20;357:151583. doi: 10.1016/j.ijbiomac.2026.151583. PMID:41865923 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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