28lx

crystal structure of Dpo31, of a tail-spike protein with depolymerase activity identified in a marine podovirus

spinqualitylabelsall models 28lx, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 28lx is a 1 chain structure with sequence from Cobetia phage Carin1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Ligands: EDO, MG, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Marine phages are, through the infection of their bacterial hosts, key regulators of microbiome and carbon fluxes in the ocean. Despite their important role, the specific molecular mechanisms that underlie infection are so far understudied. Previously, the podovirus Cobetia marina virus 1 (Carin-1), which infects the marine gamma-proteobacterium C. marina, was shown to display exopolysaccharide depolymerase activity. This activity is likely to mediate degradation of the host capsule to facilitate access to the bacterial membrane receptor, but no corresponding gene could be annotated in the genome of Carin-1 by comparative genomics. Biochemical characterization enabled assignment of this activity to Dpo31, a protein sharing less than 10% sequence identity with any characterized protein. Here, we report the structural domain organization and biochemical characterization of Dpo31, revealing an overall structure that is analogous to podovirus tail-spike proteins, allowing us to locate the depolymerase activity to the D3 domain and to identify original structural features that explain the absence of detectable similarity at the primary-sequence level. Biochemical and structural characterization of a tail-spike protein with depolymerase activity identified in a marine podovirus.,Sirigu S, Roret T, Mocaer PY, Larocque R, Jouanneau D, Legrand P, Baudoux AC, Czjzek M Acta Crystallogr D Struct Biol. 2026 Jul 1;82(Pt 7):785-799. doi: , 10.1107/S2059798326005425. Epub 2026 Jun 17. PMID:42308020 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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