| Structural highlights
Function
DNAB_BACSU Helps DnaI load the DnaC replicative helicase onto single-stranded (ss)DNA (PubMed:12718886). During DNA replication from the origin of replication (oriC) in the DNA replisome, DnaD is required after DnaA, before DnaB and before subsequent helicase DnaC loading (PubMed:15186423, PubMed:19968790). Component of the replication restart primosome, which reloads the replicative helicase on sites other than oriC (PubMed:11585815). DnaB, DnaD and DnaI may also be required for a PriA-independent pathway of replication fork restart (PubMed:11679082). DnaB and DnaD work together to allow DnaB access to ssDNA (PubMed:15686560). DNA replication at oriC might originate on the inner face of the cell membrane; DnaB is essential for both replication initiation and cell membrane attachment of the origin region of the chromosome and plasmids (PubMed:6771760, PubMed:3027671, PubMed:3027697). Weakly binds ssDNA (PubMed:11585815, PubMed:15686560, PubMed:32817095), preferentially binds double-stranded (ds)DNA (PubMed:32817095), and replication fork-like substrates (PubMed:11585815). Remodels DNA, laterally compacts supercoiled plasmid and linear DNA, forms beads along the dsDNA (PubMed:16002087). Together DnaB and DnaD form bipolar complexes on plasmid DNA (PubMed:16002087). DnaB and DnaD are also required to load helicase on the repN plasmid origin of replication (oriN) (PubMed:36416272).[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
References
- ↑ Bruand C, Farache M, McGovern S, Ehrlich SD, Polard P. DnaB, DnaD and DnaI proteins are components of the Bacillus subtilis replication restart primosome. Mol Microbiol. 2001 Oct;42(1):245-55. doi: 10.1046/j.1365-2958.2001.02631.x. PMID:11679082 doi:https://dx.doi.org/10.1046/j.1365-2958.2001.02631.x
- ↑ Velten M, McGovern S, Marsin S, Ehrlich SD, Noirot P, Polard P. A two-protein strategy for the functional loading of a cellular replicative DNA helicase. Mol Cell. 2003 Apr;11(4):1009-20. doi: 10.1016/s1097-2765(03)00130-8. PMID:12718886 doi:https://dx.doi.org/10.1016/s1097-2765(03)00130-8
- ↑ Rokop ME, Auchtung JM, Grossman AD. Control of DNA replication initiation by recruitment of an essential initiation protein to the membrane of Bacillus subtilis. Mol Microbiol. 2004 Jun;52(6):1757-67. doi: 10.1111/j.1365-2958.2004.04091.x. PMID:15186423 doi:https://dx.doi.org/10.1111/j.1365-2958.2004.04091.x
- ↑ Zhang W, Carneiro MJ, Turner IJ, Allen S, Roberts CJ, Soultanas P. The Bacillus subtilis DnaD and DnaB proteins exhibit different DNA remodelling activities. J Mol Biol. 2005 Aug 5;351(1):66-75. doi: 10.1016/j.jmb.2005.05.065. PMID:16002087 doi:https://dx.doi.org/10.1016/j.jmb.2005.05.065
- ↑ Smits WK, Goranov AI, Grossman AD. Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo. Mol Microbiol. 2010 Jan;75(2):452-61. doi: 10.1111/j.1365-2958.2009.06999.x. Epub , 2009 Dec 4. PMID:19968790 doi:https://dx.doi.org/10.1111/j.1365-2958.2009.06999.x
- ↑ Ogasawara N, Moriya S, Mazza PG, Yoshikawa H. Nucleotide sequence and organization of dnaB gene and neighbouring genes on the Bacillus subtilis chromosome. Nucleic Acids Res. 1986 Dec 22;14(24):9989-99. doi: 10.1093/nar/14.24.9989. PMID:3027671 doi:https://dx.doi.org/10.1093/nar/14.24.9989
- ↑ Hoshino T, McKenzie T, Schmidt S, Tanaka T, Sueoka N. Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment. Proc Natl Acad Sci U S A. 1987 Feb;84(3):653-7. doi: 10.1073/pnas.84.3.653. PMID:3027697 doi:https://dx.doi.org/10.1073/pnas.84.3.653
- ↑ Matthews LA, Simmons LA. Regulation of DNA Binding and High-Order Oligomerization of the DnaB Helicase Loader. J Bacteriol. 2020 Oct 8;202(21):e00286-20. doi: 10.1128/JB.00286-20. Print 2020 , Oct 8. PMID:32817095 doi:https://dx.doi.org/10.1128/JB.00286-20
- ↑ Winterhalter C, Stevens D, Fenyk S, Pelliciari S, Marchand E, Soultanas P, Ilangovan A, Murray H. SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment during Bacillus subtilis sporulation. Nucleic Acids Res. 2023 May 22;51(9):4302-4321. doi: 10.1093/nar/gkac1060. PMID:36416272 doi:https://dx.doi.org/10.1093/nar/gkac1060
- ↑ Winston S, Sueoka N. DNA-membrane association is necessary for initiation of chromosomal and plasmid replication in Bacillus subtilis. Proc Natl Acad Sci U S A. 1980 May;77(5):2834-8. doi: 10.1073/pnas.77.5.2834. PMID:6771760 doi:https://dx.doi.org/10.1073/pnas.77.5.2834
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