2d45
Crystal structure of the MecI-mecA repressor-operator complex
Structural highlights
FunctionMECI_STAAN Transcriptional repressor that constitutively blocks the transcription of the gene for the penicillin-binding protein MecA. Binds palindromic DNA with the sequence 5'-TACA-[AT]-N-TGTA-3'. Regulates genes involved in antibiotic resistance. Binds DNA as a dimer. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe dimeric repressor MecI regulates the mecA gene that encodes the penicillin-binding protein PBP-2a in methicillin-resistant Staphylococcus aureus (MRSA). MecI is similar to BlaI, the repressor for the blaZ gene of beta-lactamase. MecI and BlaI can bind to both operator DNA sequences. The crystal structure of MecI in complex with the 32 base-pair cognate DNA of mec was determined to 3.8 A resolution. MecI is a homodimer and each monomer consists of a compact N-terminal winged-helix domain, which binds to DNA, and a loosely packed C-terminal helical domain, which intertwines with its counter-monomer. The crystal contains horizontal layers of virtual DNA double helices extending in three directions, which are separated by perpendicular DNA segments. Each DNA segment is bound to two MecI dimers. Similar to the BlaI-mec complex, but unlike the MecI-bla complex, the MecI repressors bind to both sides of the mec DNA dyad that contains four conserved sequences of TACA/TGTA. The results confirm the up-and-down binding to the mec operator, which may account for cooperative effect of the repressor. Structure of the MecI repressor from Staphylococcus aureus in complex with the cognate DNA operator of mec.,Safo MK, Ko TP, Musayev FN, Zhao Q, Wang AH, Archer GL Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):320-4. Epub 2006 Mar 25. PMID:16582476[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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