2d6y
Crystal Structure of transcriptional factor SCO4008 from Streptomyces coelicolor A3(2)
Structural highlights
FunctionHTHR_STRCO Probably regulates the expression of its own gene and the adjacent SCO4007 gene by binding to two operator sites in the SCO4007-SCO4008 intergenic region.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedSCO4008 from Streptomyces coelicolor A3(2) is a member of the TetR family. However, its precise function is not yet clear. In this study, the crystal structure of SCO4008 was determined at a resolution of 2.3A, and its DNA-binding properties were analyzed. Crystal structure analysis showed that SCO4008 forms an Omega-shaped homodimer in which the monomer is composed of an N-terminal DNA-binding domain containing a helix-turn-helix and a C-terminal dimerization and regulatory domain possessing a ligand-binding cavity. The genomic systematic evolution of ligands by exponential enrichment and electrophoretic mobility shift assay revealed that four SCO4008 dimers bind to the two operator regions located between sco4008 and sco4007, a secondary transporter belonging to the major facilitator superfamily. Ligand screening analysis showed that SCO4008 recognizes a wide range of structurally dissimilar cationic and hydrophobic compounds. These results suggested that SCO4008 is a transcriptional repressor of sco4007 responsible for the multidrug resistance system in S. coelicolor A3(2). SCO4008, a Putative TetR Transcriptional Repressor from Streptomyces coelicolor A3(2), Regulates Transcription of sco4007 by Multidrug Recognition.,Hayashi T, Tanaka Y, Sakai N, Okada U, Yao M, Watanabe N, Tamura T, Tanaka I J Mol Biol. 2013 Sep 23;425(18):3289-300. doi: 10.1016/j.jmb.2013.06.013. Epub, 2013 Jul 2. PMID:23831227[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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