2if9

Crystal Structure of SV40 T-antigen origin binding domain disulfide-linked dimer

spinqualitylabelsall models 2if9, resolution 2.59Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2if9 is a 2 chain structure with sequence from Macaca mulatta polyomavirus 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.586Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q98ZP7_SV40 Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed DNA replication is initiated upon binding of "initiators" to origins of replication. In simian virus 40 (SV40), the core origin contains four pentanucleotide binding sites organized as pairs of inverted repeats. Here we describe the crystal structures of the origin binding domain (obd) of the SV40 large T-antigen (T-ag) both with and without a subfragment of origin-containing DNA. In the co-structure, two T-ag obds are oriented in a head-to-head fashion on the same face of the DNA, and each T-ag obd engages the major groove. Although the obds are very close to each other when bound to this DNA target, they do not contact one another. These data provide a high-resolution structural model that explains site-specific binding to the origin and suggests how these interactions help direct the oligomerization events that culminate in assembly of the helicase-active dodecameric complex of T-ag. The crystal structure of the SV40 T-antigen origin binding domain in complex with DNA.,Meinke G, Phelan P, Moine S, Bochkareva E, Bochkarev A, Bullock PA, Bohm A PLoS Biol. 2007 Feb;5(2):e23. PMID:17253903 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Large T Antigen

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