2jjb

Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose

spinqualitylabelsall models 2jjb, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2jjb is a 4 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.9Å Ligands: 3CU, EDO, GLC, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TREA_ECOLI Provides the cells with the ability to utilize trehalose at high osmolarity by splitting it into glucose molecules that can subsequently be taken up by the phosphotransferase-mediated uptake system.[HAMAP-Rule:MF_01060] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Total synthesis of naturally occurring casuarine (1) and the first total synthesis of casuarine 6-O-alpha-glucoside (2) were achieved through complete stereoselective nitrone cycloaddition, Tamao-Fleming oxidation and selective alpha-glucosylation as key steps. Biological assays of the two compounds proved their strong and selective inhibitory properties towards glucoamylase NtMGAM and trehalase Tre37A, respectively, which place them among the most powerful inhibitors of these enzymes. The structural determination of the complexes of NtMGAM with 1 and of Tre37A with 2 revealed interesting similarities in the catalytic sites of these two enzymes which belong to different families and clans. Total Syntheses of Casuarine and Its 6-O-alpha-Glucoside: Complementary Inhibition towards Glycoside Hydrolases of the GH31 and GH37 Families.,Cardona F, Parmeggiani C, Faggi E, Bonaccini C, Gratteri P, Sim L, Gloster TM, Roberts S, Davies GJ, Rose DR, Goti A Chemistry. 2009 Jan 2. PMID:19123216 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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