2k2s
structure of the MIC1-GLD/MIC6-EGF complex from Toxoplasma gondii
Structural highlights
FunctionMIC1_TOXGO Adhesin. Required for attachment of the parasite to the host cell prior to invasion. Ensures correct folding of MIC6 and transport of the MIC6-MIC1-MIC4 complex into the micronemes.[1] [2] [3] [4] [5] Publication Abstract from PubMedThe obligate intracellular parasite Toxoplasma gondii, a member of the phylum Apicomplexa that includes Plasmodium spp., is one of the most widespread parasites and the causative agent of toxoplasmosis. Adhesive complexes composed of microneme proteins (MICs) are secreted onto the parasite surface from intracellular stores and fulfil crucial roles in host-cell recognition, attachment and penetration. Here, we report the high-resolution solution structure of a complex between two crucial MICs, TgMIC6 and TgMIC1. Furthermore, we identify two analogous interaction sites within separate epidermal growth factor-like (EGF) domains of TgMIC6-EGF2 and EGF3-and confirm that both interactions are functional for the recognition of host cell receptor in the parasite, using immunofluorescence and invasion assays. The nature of this new mode of recognition of the EGF domain and its abundance in apicomplexan surface proteins suggest a more generalized means of constructing functional assemblies by using EGF domains with highly specific receptor-binding properties. Structural insights into microneme protein assembly reveal a new mode of EGF domain recognition.,Sawmynaden K, Saouros S, Friedrich N, Marchant J, Simpson P, Bleijlevens B, Blackman MJ, Soldati-Favre D, Matthews S EMBO Rep. 2008 Nov;9(11):1149-55. Epub 2008 Sep 26. PMID:18818666[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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