2ldo

Solution structure of triheme cytochrome PpcA from Geobacter sulfurreducens reveals the structural origin of the redox-Bohr effect

spinqualitylabelsall models 2ldo Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2ldo is a 1 chain structure with sequence from Geobacter sulfurreducens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, models Ligands: HEC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8GGK7_GEOSN Publication Abstract from PubMed Geobacter sulfurreducens (Gs) can transfer electrons to the exterior of its cells, a property that makes it a preferential candidate for the development of biotechnological applications. Its genome encodes for over one hundred cytochromes and despite their abundance and key functional roles, to date there is no structural information for these proteins in solution. The trihaem cytochrome PpcA has a crucial role in the conversion of electronic energy into proton motive force, a fundamental step for ATP synthesis in the presence of extracellular electron acceptors. In this work, 15N-labelled PpcA was produced and NMR spectroscopy was used to determine its solution structure in the fully reduced state, its backbone dynamics and the pH-dependent conformational changes. The structure obtained is well defined, with an average pairwise root-mean-square deviation of 0.25 A for the backbone atoms and 0.99 A for all heavy atoms, and constitutes the first solution structure of a Gs cytochrome. The redox-Bohr centre responsible for controlling the electron/proton transfer was identified, as well as the putative interacting regions between PpcA and its redox partners. The solution structure of PpcA will constitute the foundation for studies aimed at mapping out in detail these interacting regions. Revealing the structural origin of the redox-Bohr effect: the first solution structure of a cytochrome from Geobacter sulfurreducens.,Morgado L, Paixao VB, Schiffer M, Pokkuluri PR, Bruix M, Salgueiro CA Biochem J. 2011 Aug 24. PMID:21861844 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cytochrome C 3D structures

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