2mi5

Structure of insect-specific sodium channel toxin mu-Dc1a

spinqualitylabelsall models 2mi5 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2mi5 is a 1 chain structure with sequence from Diguetia canities. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 20 models Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TXI92_DIGCA Publication Abstract from PubMed beta-Diguetoxin-Dc1a (Dc1a) is a toxin from the desert bush spider Diguetia canities that incapacitates insects at concentrations that are non-toxic to mammals. Dc1a promotes opening of German cockroach voltage-gated sodium (Nav) channels (BgNav1), whereas human Nav channels are insensitive. Here, by transplanting commonly targeted S3b-S4 paddle motifs within BgNav1 voltage sensors into Kv2.1, we find that Dc1a interacts with the domain II voltage sensor. In contrast, Dc1a has little effect on sodium currents mediated by PaNav1 channels from the American cockroach even though their domain II paddle motifs are identical. When exploring regions responsible for PaNav1 resistance to Dc1a, we identified two residues within the BgNav1 domain II S1-S2 loop that when mutated to their PaNav1 counterparts drastically reduce toxin susceptibility. Overall, our results reveal a distinct region within insect Nav channels that helps determine Dc1a sensitivity, a concept that will be valuable for the design of insect-selective insecticides. A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a.,Bende NS, Dziemborowicz S, Mobli M, Herzig V, Gilchrist J, Wagner J, Nicholson GM, King GF, Bosmans F Nat Commun. 2014 Jul 11;5:4350. doi: 10.1038/ncomms5350. PMID:25014760 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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