2mme

Hybrid structure of the Shigella flexneri MxiH Type three secretion system needle

Loading... Status: waiting for Mol* initialization Structural highlights 2mme is a 29 chain structure with sequence from Shigella flexneri. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy , Hybrid , Solid-state NMR, Resolution 7.7Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q6XVY0_SHIFL Publication Abstract from PubMed We introduce a general hybrid approach for determining the structures of supramolecular assemblies. Cryo-electron microscopy (cryo-EM) data define the overall envelope of the assembly and rigid-body orientation of the subunits while solid-state nuclear magnetic resonance (ssNMR) chemical shifts and distance constraints define the local secondary structure, protein fold and inter-subunit interactions. Finally, Rosetta structure calculations provide a general framework to integrate the different sources of structural information. Combining a 7.7-A cryo-EM density map and 996 ssNMR distance constraints, the structure of the type-III secretion system needle of Shigella flexneri is determined to a precision of 0.4 A. The calculated structures are cross-validated using an independent data set of 691 ssNMR constraints and scanning transmission electron microscopy measurements. The hybrid model resolves the conformation of the non-conserved N terminus, which occupies a protrusion in the cryo-EM density, and reveals conserved pore residues forming a continuous pattern of electrostatic interactions, thereby suggesting a mechanism for effector protein translocation. High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.,Demers JP, Habenstein B, Loquet A, Kumar Vasa S, Giller K, Becker S, Baker D, Lange A, Sgourakis NG Nat Commun. 2014 Sep 29;5:4976. doi: 10.1038/ncomms5976. PMID:25264107 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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