2mna

The structural basis of DNA binding by the single-stranded DNA-binding protein from Sulfolobus solfataricus

spinqualitylabelsall models 2mna Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2mna is a 2 chain structure with sequence from Saccharolobus solfataricus P2. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SSB_SACS2 Binds to ssDNA, binding approximately 5 nucleotides per monomer. Binding my be slightly cooperative. Inhibits the endonuclease activity of XPF. Publication Abstract from PubMed Canonical single-stranded DNA binding proteins (SSBs) from the Oligosaccharide/Oligonucleotide Binding (OB) domain family are present in all known organisms and are critical for DNA replication, recombination and repair. The SSB from the hyperthermophilic crenarchaeote Sulfolobus solfataricus (SsoSSB) has a 'simple' domain organisation consisting of a single DNA binding OB fold coupled to a flexible C-terminal tail, in contrast to other SSBs in this family that incorporate up to four OB domains. Despite the large differences in the domain organisation within the SSB family, the structure of the OB domain is remarkably similar in all cellular life forms. However, there are significant differences in the molecular mechanism of single-stranded DNA (ssDNA) binding. We have determined the structure of the SsoSSB OB domain bound to ssDNA by NMR spectroscopy. We reveal that ssDNA recognition is modulated by base-stacking of three key aromatic residues, in contrast to the OB domains of human RPA and the recently discovered human homologue of SsoSSB, hSSB1. We also demonstrate that SsoSSB binds ssDNA with a footprint of five bases and with a defined binding polarity. These data elucidate the structural basis of DNA binding and shed light onto the molecular mechanism by which these 'simple' SSBs interact with ssDNA. The structural basis of DNA binding by the single stranded DNA binding protein from Sulfolobus solfataricus.,Gamsjaeger R, Kariawasam R, Gimenez AX, Touma C, McIlwain E, Bernardo RE, Shepherd NE, Ataide SF, Dong Q, Richard DJ, White MF, Cubeddu L Biochem J. 2014 Nov 4. PMID:25367669 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Single-stranded DNA-binding protein 3D structures

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