2mz8

Solution NMR structure of Salmonella Typhimurium transcriptional regulator protein Crl

spinqualitylabelsall models 2mz8 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2mz8 is a 1 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CRL_SALTY Binds to the sigma-S subunit of RNA polymerase, activating expression of sigma-S-regulated genes, such as the csgBAC operon encoding the subunits of curli proteins, and BcsA, involved in cellulose biosynthesis. Publication Abstract from PubMed In many Gram-negative bacteria, including Salmonella enterica serovar Typhimurium (S. Typhimurium), the sigma factor RpoS/sigma(S) accumulates during stationary phase of growth, and associates with the core RNA polymerase enzyme (E) to promote transcription initiation of genes involved in general stress resistance and starvation survival. Whereas sigma factors are usually inactivated upon interaction with anti-sigma proteins, sigma(S) binding to the Crl protein increases sigma(S) activity by favouring its association to E. Taking advantage of evolution of the sigma(S) sequence in bacterial species that do not contain a crl gene, like Pseudomonas aeruginosa, we identified and assigned a critical arginine residue in sigma(S) to the S. Typhimurium sigma(S)-Crl binding interface. We solved the solution structure of S. Typhimurium Crl by NMR and used it for NMR binding assays with sigma(S) and to generate in silico models of the sigma(S)-Crl complex constrained by mutational analysis. The sigma(S)-Crl models suggest that the identified arginine in sigma(S) interacts with an aspartate of Crl that is required for sigma(S) binding and is located inside a cavity enclosed by flexible loops, which also contribute to the interface. This study provides the basis for further structural investigation of the sigma(S)-Crl complex. Binding interface between the Salmonella sigma(S)/RpoS subunit of RNA polymerase and Crl: hints from bacterial species lacking crl.,Cavaliere P, Sizun C, Levi-Acobas F, Nowakowski M, Monteil V, Bontems F, Bellalou J, Mayer C, Norel F Sci Rep. 2015 Sep 4;5:13564. doi: 10.1038/srep13564. PMID:26338235 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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