2n2y

Solution structure of the meiosis-expressed gene 1 (Meig1)

spinqualitylabelsall models 2n2y Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2n2y is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function MEIG1_MOUSE Essential for spermiogenesis. Publication Abstract from PubMed The product of the meiosis-expressed gene 1 (MEIG1) is found in the cell bodies of spermatocytes and recruited to the manchette, a structure unique to elongating spermatids, by Parkin co-regulated gene (PACRG). This complex is essential for targeting cargo to the manchette during sperm flagellum assembly. Here we show that MEIG1 adopts a unique fold that provides a large surface for interacting with other proteins. We mutated 12 exposed and conserved amino acids and show that four of these mutations (W50A, K57E, F66A, Y68A) dramatically reduce binding to PACRG. These four amino acids form a contiguous hydrophobic patch on one end of the protein. Furthermore, each of these four mutations diminishes the ability of MEIG1 to stabilize PACRG when expressed in bacteria. Together these studies establish the unique structure and key interaction surface of MEIG1 and provide a framework to explore how MEIG1 recruits proteins to build the sperm tail. Dissecting the structural basis of MEIG1 interaction with PACRG.,Li W, Walavalkar NM, Buchwald WA, Teves ME, Zhang L, Liu H, Bilinovich S, Peterson DL, Strauss Iii JF, Williams DC Jr, Zhang Z Sci Rep. 2016 Jan 4;6:18278. doi: 10.1038/srep18278. PMID:26726850 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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