2n50
Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis
Structural highlights
FunctionQ82ZE9_ENTFA Carrier of the growing fatty acid chain in fatty acid biosynthesis.[RuleBase:RU003545] Publication Abstract from PubMedEnterococcus faecalis is a Gram-positive, commensal bacterium that lives in the gastrointestinal tracts of humans and other mammals. It causes severe infections because of high antibiotic resistance. E. faecalis can endure extremes of temperature and pH. Acyl carrier protein (ACP) is a key element in the biosynthesis of fatty acids responsible for acyl group shuttling and delivery. In this study, to understand the origin of high thermal stabilities of E. faecalis ACP (Ef-ACP), its solution structure was investigated for the first time. CD experiments showed that the melting temperature of Ef-ACP is 78.8 degrees C, which is much higher than that of Escherichia coli ACP (67.2 degrees C). The overall structure of Ef-ACP shows the common ACP folding pattern consisting of four alpha-helices (helix I (residues 3-17), helix II (residues 39-53), helix III (residues 60-64), and helix IV (residues 68-78)) connected by three loops. Unique Ef-ACP structural features include a hydrophobic interaction between Phe(45) in helix II and Phe(18) in the alpha1alpha2 loop and a hydrogen bonding between Ser(15) in helix I and Ile(20) in the alpha1alpha2 loop, resulting in its high thermal stability. Phe(45)-mediated hydrophobic packing may block acyl chain binding subpocket II entry. Furthermore, Ser(58) in the alpha2alpha3 loop in Ef-ACP, which usually constitutes a proline in other ACPs, exhibited slow conformational exchanges, resulting in the movement of the helix III outside the structure to accommodate a longer acyl chain in the acyl binding cavity. These results might provide insights into the development of antibiotics against pathogenic drug-resistant E. faecalis strains. Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis.,Park YG, Jung MC, Song H, Jeong KW, Bang E, Hwang GS, Kim Y J Biol Chem. 2016 Jan 22;291(4):1692-702. doi: 10.1074/jbc.M115.674408. Epub 2015, Dec 2. PMID:26631734[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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