2n8k

Chemical Shift Assignments and Structure Determination for spider toxin, U33-theraphotoxin-Cg1c

spinqualitylabelsall models 2n8k Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2n8k is a 1 chain structure with sequence from Chilobrachys guangxiensis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 20 models Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function JZT70_CHIGU Publication Abstract from PubMed Spiders are one of the most successful venomous animals, with more than 48,000 described species. Most spider venoms are dominated by cysteine-rich peptides with a diverse range of pharmacological activities. Some spider venoms contain thousands of unique peptides, but little is known about the mechanisms used to generate such complex chemical arsenals. We used an integrated transcriptomic, proteomic, and structural biology approach to demonstrate that the lethal Australian funnel-web spider produces 33 superfamilies of venom peptides and proteins. Twenty-six of the 33 superfamilies are disulfide-rich peptides, and we show that 15 of these are knottins that contribute >90% of the venom proteome. NMR analyses revealed that most of these disulfide-rich peptides are structurally related and range in complexity from simple to highly elaborated knottin domains, as well as double-knot toxins, that likely evolved from a single ancestral toxin gene. Structural venomics reveals evolution of a complex venom by duplication and diversification of an ancient peptide-encoding gene.,Pineda SS, Chin YK, Undheim EAB, Senff S, Mobli M, Dauly C, Escoubas P, Nicholson GM, Kaas Q, Guo S, Herzig V, Mattick JS, King GF Proc Natl Acad Sci U S A. 2020 May 26;117(21):11399-11408. doi: , 10.1073/pnas.1914536117. Epub 2020 May 12. PMID:32398368 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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