2q9t

High-resolution structure of the DING protein from Pseudomonas fluorescens

spinqualitylabelsall models 2q9t, resolution 1.43Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2q9t is a 1 chain structure with sequence from Pseudomonas fluorescens SBW25. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.43Å Ligands: ACT, EDO, GOL, PO4, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D0VWY2_PSEFS Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed A recombinant DING protein from Pseudomonas fluorescens has been previously shown to have a phosphate-binding site, and to be mitogenic for human cells. Here we report the three-dimensional structure of the protein, confirming a close similarity to the "Venus flytrap" structure seen in other human and bacterial phosphate-binding proteins. Site-directed mutagenesis confirms the role of a key residue involved in phosphate binding, and that the mitogenic activity is not dependent on this property. Deletion of one of the two hinged domains that constitute the Venus flytrap also eliminates phosphate binding whilst enhancing mitogenic activity. Structure-function relationships in a bacterial DING protein.,Ahn S, Moniot S, Elias M, Chabriere E, Kim D, Scott K FEBS Lett. 2007 Jul 24;581(18):3455-60. Epub 2007 Jun 27. PMID:17612529 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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