2wwb

CRYO-EM STRUCTURE OF THE MAMMALIAN SEC61 COMPLEX BOUND TO THE ACTIVELY TRANSLATING WHEAT GERM 80S RIBOSOME

Loading... Status: waiting for Mol* initialization Structural highlights 2wwb is a 10 chain structure with sequence from Canis lupus familiaris and Triticum aestivum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 6.48Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function S61A1_CANLF Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found that in both idle and active states, the Sec complex is not oligomeric and interacts mainly via two cytoplasmic loops with the universal ribosomal adaptor site. In the active state, the ribosomal tunnel and a central pore of the monomeric PCC were occupied by the nascent chain, contacting loop 6 of the Sec complex. This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation. Structure of monomeric yeast and Mammalian sec61 complexes interacting with the translating ribosome.,Becker T, Bhushan S, Jarasch A, Armache JP, Funes S, Jossinet F, Gumbart J, Mielke T, Berninghausen O, Schulten K, Westhof E, Gilmore R, Mandon EC, Beckmann R Science. 2009 Dec 4;326(5958):1369-73. Epub 2009 Oct 29. PMID:19933108 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosomal protein L17 Ribosomal protein L19 Ribosomal protein L23 Ribosomal protein L25 Ribosomal protein L26 Ribosomal protein L31 Ribosomal protein L35 Ribosomal protein L39

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