2x9c

Crystal structure of a soluble PrgI mutant from Salmonella Typhimurium

spinqualitylabelsall models 2x9c, resolution 2.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2x9c is a 2 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.45Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PRGI_SALTY Required for invasion of epithelial cells. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Pathogenic Gram-negative bacteria use a type three secretion system (TTSS) to deliver virulence factors into host cells. Although the order in which proteins incorporate into the growing TTSS is well described, the underlying assembly mechanisms are still unclear. Here we show that the TTSS needle protomer refolds spontaneously to extend the needle from the distal end. We developed a functional mutant of the needle protomer from Shigella flexneri and Salmonella typhimurium to study its assembly in vitro. We show that the protomer partially refolds from alpha-helix into beta-strand conformation to form the TTSS needle. Reconstitution experiments show that needle growth does not require ATP. Thus, like the structurally related flagellar systems, the needle elongates by subunit polymerization at the distal end but requires protomer refolding. Our studies provide a starting point to understand the molecular assembly mechanisms and the structure of the TTSS at atomic level. Protein refolding is required for assembly of the type three secretion needle.,Poyraz O, Schmidt H, Seidel K, Delissen F, Ader C, Tenenboim H, Goosmann C, Laube B, Thunemann AF, Zychlinsky A, Baldus M, Lange A, Griesinger C, Kolbe M Nat Struct Mol Biol. 2010 Jul;17(7):788-92. Epub 2010 Jun 13. PMID:20543831 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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