2xw7

Structure of Mycobacterium smegmatis putative reductase MS0308

spinqualitylabelsall models 2xw7, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2xw7 is a 2 chain structure with sequence from Mycolicibacterium smegmatis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: NA, NDP, PG4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0QP85_MYCS2 Publication Abstract from PubMed Mycobacterium tuberculosis, the most successful bacterial pathogen, causes tuberculosis, a disease that still causes more than 2 million deaths per year. Arylamine N-acetyltransferase is an enzyme that is conserved in most Mycobacterium spp. The nat gene belongs to an operon that is important for the intracellular survival of M. tuberculosis within macrophages. The nat operon in Mycobacterium smegmatis and other fast-growing mycobacterial species has a unique organization containing genes with uncharacterized function. Here, we describe the biochemical, biophysical and structural characterization of the MSMEG_0308 gene product (MS0308) of the M. smegmatis nat operon. While characterizing the function of MS0308, we validated the oxidoreductase property; however, we found that the enzyme was not utilizing dihydrofolate as its substrate, hence we first report that MS0308 is not a dihydrofolate reductase, as annotated in the genome. The structure of this oxidoreductase was solved at 2.0 A in complex with the cofactor NADPH and has revealed the hydrophobic pocket where the endogenous substrate binds. Characterization of an oxidoreductase from the arylamine N-acetyltransferase operon in Mycobacterium smegmatis.,Evangelopoulos D, Cronin N, Daviter T, Sim E, Keep NH, Bhakta S FEBS J. 2011 Dec;278(24):4824-32. doi: 10.1111/j.1742-4658.2011.08382.x. Epub, 2011 Oct 31. PMID:21972977 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Dihydrofolate reductase 3D structures

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